By far the best proof is experience.  

Sir Francis Bacon (1561 - 1626)

Gain

Gain is the same as the contrast control on your TV. It is a multiplication of the signal. In math terms, it controls the slope of the exposure/time curve. The camera should normally be operated at the lowest gain possible, because gain not only multiplies the signal, but also multiplies the noise. Gain comes in very handy when you require a short exposure (say, because the object is moving and you do not want any blur), but do not have adequate lighting. In this situation the gain can be increased so that the image signal is strong. 

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